Analysis Note

ControlEmbryonic chicken brain

Application

Function blocking

Western blot: Antibody recognizes a 220-240kDa band in reduced westerns; antibody reacts best with chicken, followed by human (100X less reactive). Mouse reactivity under reduced conditions is poor.

Immunohistochemistry: frozen and paraffin embedded tissues; citrate treatment useful for paraffin tissues.

Optimal working dilutions must be determined by the end user.

Anti-Tenascin Antibody is an antibody against Tenascin for use in IH, IH(P).

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified tenascin from embryonic chicken brain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1951

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified using immobilized chicken tenascin. Provided as a liquid in PBS, containing no preservatives.

ImmunoAffinity Purified

Specificity

Reactive with tenascin from chicken and human by immunohistochemistry. Under reducing conditions, reactivity to chicken is approximately 100X greater than reactivity with human tenascin. Reactivity with mouse tenascin under reducing conditions is poor, but reacts with mouse in paraffin embedded sections.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.